Viral DNA extraction and purification magnetic beads kit
This kit allows for extraction and purification of viral DNA from serum, plasma, urine, lymph, cell culture supernatants or from a variety of viral-containing fluids. Using proprietary Viral Lysis solution, viral DNA can be efficiently extracted and then purified using magnetic beads, yielding high pure viral DNA with a ratio of OD260/280 between 1.75 and 1.85. The recovered DNA size can be up to 60kb.
The kit will work with a 48 well round bottom plates if a special magnetic frame is used.
The kit can also be used with a variety of automatic nucleic acid extraction instruments or workstations. |
Catalog # |
Si-Mag-VD1 |
Precautions |
- Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling.
- Avoid freeze/thaw cycles and centrifugation which could damage the beads.
- Proteinase K solution should be stored at 4°C.
- Bring frozen viral samples to room temperature before extraction.
- Vortex samples for about 10 seconds before adding magnetic beads.
- Vortex beads for about 10 seconds and mix them well with DNA containing material to ensure best performance.
- Elute DNA from the beads completely.
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Kit Components included |
- Si-Mag magnetic beads 3 mL
- Proteinase K solution 2 mL
- Viral Lysis solution 40 mL
- Wash solution 32 mL (mix with 32 mL of Isopropanol before use)
- Elution Buffer 10 mL
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Materials needed but not provided with the kit |
- 80% Ethanol in water.
- Si-Mag Magnet (sold separately) or other magnetic racks compatible with vials used.
- Isopropanol (ACS grade).
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Storage |
Magnetic beads and Proteinase K should be stored at 2-8°C but other kit reagents need to be stored at room temperature. |
Protocol |
- Preparation of sample. Add 200 uL of viral fluid sample, 400 uL of viral lysis solution, and 20 uL of Proteinase K solution into a clean Eppendorf tube. Vortex for 10 seconds then incubate for 10 min at 58°C (option: or incubate at room temperature for 2 and 5 min and repeat vortexing).
- Add 200 uL of isopropanol and 30 uL magnetic beads to the tube. Vortex for 30 seconds and then incubate for 2-3 min at room temperature.
- Put the Eppendorf tube onto the Si-Mag magnet rack for 20 seconds. Make sure the beads are collected at the bottom of the tube.
- Remove supernatant by holding the magnet rack upside down or by pipetting.
- Wash the beads with 600 uL of wash solution. Vortex the tube to mix well.
- Wash the beads with 600 uL of 80% ethanol for twice and repeat Steps 3-4.
- Dry the beads at 45°C for 5-10 min leaving the tube open. Do not over-dry the beads.
- Elute the DNA from beads with 50-100 uL of elution buffer, incubate at 60°C for 5 min and then vortex at full speed for 1 min. Wait for 5 min and repeat the vortexing again.
- Remove beads by using magnet rack, pipette DNA out and transfer to a clean tube.
- Store purified DNA at -20°C for long-term storage.
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